《植物生理学报》 2015, 51(6): 797-805

植物组织特异性基因表达技术及其应用

方彦昊, 南文斌, 梁永书, 张汉马*

重庆师范大学生命科学学院, 重庆市植物环境适应分子生物学重庆重点实验室, 重庆 401331

通信作者：张汉马；E-mail: hanmazhang@126.com；Tel: 023-65912976

摘　要：

研究表明在植物以及其它多细胞生物体中, 有些基因只在生长发育的特定阶段、器官、组织或细胞中表达。对这类基因的研究一方面有助于我们了解该基因自身的功能, 另一方面也可以作为工具应用到其他相关研究当中, 例如利用植物中具特异表达特性的基因的调控元件将特定基因在特定器官、组织或细胞中表达的技术, 即植物组织特异性基因表达技术。本文主要介绍目前被广泛使用的两种植物组织特异性基因表达技术, 即组织特异性启动子驱动法和GAL4/UAS激活标签法及其应用, 为今后的研究工作提供一定的参考。

关键词：基因; 启动子; 组织特异性; GAL4/UAS

收稿：2015-01-19   修定：2015-05-12

资助：“973”计划前期研究专项(2014CB160306)、重庆市教委创新团队建设基金(KJTD201307)和重庆师范大学引进人才启动基金项目(12XLR36)。

Plant Tissue-Specific Gene Expression System and Its Applications

FANG Yan-Hao, NAN Wen-Bin, LIANG Yong-Shu, ZHANG Han-Ma*

Chongqing Key Laboratory of Molecular Biology of Plants Environmental Adaptations, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China

Corresponding author: ZHANG Han-Ma; E-mail: hanmazhang@126.com; Tel: 023-65912976

Abstract:

It is known that, in plants as well as in all other multicellular organisms, there are genes that are only expressed at specific developmental stage, organs, tissues or cells. The research on those genes not only can help us to understand their functions, but also can provide valuable tools for other studies, such as developing plant tissue-specific expression systems using the regulatory elements of tissue-specifically expressed genes. In this review, we provided a brief overview of two currently wildly used methods for achieving tissue-specific expression of genes in plants, i.e. tissue-specific promoters or GAL4/UAS activation tagging method, and some examples of their applications. This brief review was intended to provide guidance to future research in this important field.

Key words: gene; promoter; tissue-specific; GAL4/UAS